Performing Blood Cell Isolation from Whole Blood Samples

Whole Blood Samples and it’s Use 

The term "whole blood samples" refers to small blood samples taken from patients or consenting donors for blood transfusions, diagnostics, treatment, and disease monitoring. It includes red blood cells (RBCs), white blood cells (WBCs), plasma, and platelets, all parts of human blood. Human blood samples from healthy donors are frequently transfused to treat patients with severe anaemia, severe bleeding, burns, and trauma.

Testing whole blood samples can reveal a wealth of data about the patient's health status. It is frequently used in clinical research to develop newer pharmacotherapeutic agents and medical diagnostic devices to treat and manage disease conditions.

Blood Cell Isolation from Human Whole Blood Samples

Apheresis is the separation of specific blood cells from whole blood samples from the patient or donor. It is primarily used for bioassays on human blood cells and for treating serious illnesses like blood disorders and blood cancers.

Blood has various densities of components. Centrifugation is used to separate blood cells from whole blood samples; as a result, causing the different blood components to precipitate into distinct layers. RBCs, which have a higher density, will form the bottom layer, followed by platelets and WBCs in the top layer.

In addition, the top layer is formed by the supernatant fluid plasma, which is then carefully separated for research purposes using a separator. A nutrient solution is added to them to keep the separated blood cell samples viable for long-term storage. The produced blood cells are then kept in sterile cryogenic storage at blood specimens repositories.

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