Detect Cross- Contamination & Other Errors with Cell Line Authentication Test

Cell lines are necessary tools for biomedical development. In the 20th century, animal cell lines were used for medicines, genetics, drug development studies, and cancer research. The cell line authentication test verifies the identity of the cells used in the research. It frequently includes confirming that cell lines are derived from the actual donor & species and that they are contaminated-free. This method produces reproducible data under the guidelines of top investigators, scientific organizations, etc. Then, a multiplex Polymerase chain reaction (PCR) generates the STR profile, followed by capillary electrophoresis on a genetic analyzer.

Why is a Cell Lines Authentication DNA Test Needed?

Three types of mistakes can be detected through the cell line authentication test.

• Cross Contamination: Environmental contaminants like fungus & dust can easily contaminate the cells under research. Maintaining a pollution-free environment in storage areas and the lab is essential to overcome this problem. However, despite the care, contamination of the cells may be inevitable. This test can help you accurately identify such mistakes.
• Misidentification: Often, a cell line of interest needs to be identified at the source. If not detected, a researcher would have continued using the same cell line for his research. They may never get the expected results. It is a common mistake while working with cell lines.
• Genetic Drift: The most vital error with a cell line is that the actual cell line may become genetically drifting with time. In such cases, the cells may lose their originality & become different cells of genetic alteration. Experts & researchers acknowledge that the latter is more harmful than the first two types of errors. You may be completely unaware that the cells are slowly changing and may continue getting wrong results over time.

When to Authenticate a Cell Line?

A cell line authenticates test involves methods like karyotyping & isoenzyme analysis. However, these techniques needed to be improved to differentiate between different cell lines. STR profiling is a simple, low-cost, & reliable method for this test. A lab should get relevant information regarding its growth characteristics, origin, & culture conditions if it receives a cell line. This test may be needed in the following conditions:

• When there is a change in the behavior of the cell
• When there is a risk of cross-contamination
• When a new cell line can be collected
• Before beginning any new experiments

Why Choose DNA Forensic Laboratory for an Accurate Cell Line Authentication Test?

Getting a Cell Lines Authentication DNA test can be an important decision, provided the sensitivity and significance of the research. 

DNA Forensics Laboratory Pvt. Ltd. uses standardized methods for cell line authentication DNA testing in India. It includes detecting the genetically drifted, cross-contaminated, or misidentified cells that invalidate research results.

We are a NABL-accredited laboratory providing accredited DNA test services, including a cell line authentication test, at reasonable prices. In addition, we have the fastest turnaround times, offering the cell line authentication test report in 12-14 business days. Furthermore, we are the only company that offers legal DNA Tests in India.

For further queries or to fix an appointment, you can call +91 8010177771 or WhatsApp us at +91 9213177771.