Human Cell Line Authentication Testing & Where is it Required?

Human Cell Line Authentication Testing & Where is it Required?

The Cell Line Authentication DNA test is the method of verifying the identity of the cells used in the research. It often includes confirming that cell lines are derived from the correct donor & species and that they are contamination-free. Therefore, cell lines are vital tools for biomedical development & research. In the early 20th century, animal cell lines were used for cancer research, medicines, drug development studies, and genetics. This process produces reproducible data under the guidelines of top researchers, scientific organizations, etc. Then, a multiplex Polymerase chain reaction generates the STR profile, followed by capillary electrophoresis on a genetic analyzer.

What is the Requirement for Cell Line Authentication Test in India?

Cells are the functional & structural units of life. Using the right cell lines is essential in genetic and biological studies and other purposes. Unfortunately, the cells are prone to alterations and biological and environmental degradation. Three kinds of errors can be detected in the cell line authentication DNA test.

• Misidentification
• Genetic Drifting
• Cross-Contamination

Misidentification- It is the most common issue in compromising the identity of the cell lines. The cell lines are misidentified at the origin. It signifies that at the collection, an incorrect cell line was collected.

Genetic Drifting- Over time, cell lines may lose their originality and may genetically drift into a different kind of cell, affecting the research/test and the final results.

Cross-Contamination- While used or stored, cell lines may get contaminated with microbes, another cell, environmental pollutants, etc.

When to Authenticate a Cell Line?

Cell line authentication tests involve techniques like isoenzyme analysis & karyotyping. However, these methods were inadequate for distinguishing between different cell lines. In contrast, STR profiling is a low-cost, easy, and reliable method for cell line authentication tests. A laboratory should get suitable information regarding its growth characteristics, origin, and culture conditions if it receives a cell line. Cell line authentication may be needed in the following states:

• When there is an alteration in the behavior of the cell
• When there is a doubt or risk of cross-contamination
• When new cell line should be collected
• Before starting any new experiments
  

How to Authenticate a Cell Line?

Short Tandem Repeat profiling establishes a unique fingerprint of every human cell line. Microsatellites are about 3-10 base pairs long & are the hypervariable parts in fungi, bacteria, mammals, fish, insects, and reptiles. STR profiling has the power to distinguish between various individuals of the same species. The number of repetitions present at the STR loci in each person differs and gives rise to a unique fingerprint. The ATCC/ANSI/standard ASN-0002 has mentioned STR profiling as the preferred human cell line authentication process.

DNA Forensics Laboratory Pvt. Ltd.- For Reliable Cell Line Authentication Tests in India

Getting a Cell Line Authentication Test in India can be a vital decision, provided the importance & sensitivity of a research/project. DNA Forensics Laboratory Pvt. Ltd. are NABL accredited laboratory offering accredited DNA testing services, including a Cell Line Authentication Test in India, at an affordable price.

They use standardized methods for a cell line authentication test. It includes detecting the genetically drifted, cross-contaminated, misidentified cells that invalidate research results. For the analysis, they use an exclusive model to develop repeated DNA for each human cell line. So that each new DNA profile stock is checked against the base profile. They are among the most reputed DNA testing companies for Cell Line Authentication Tests. Their customers are from various esteemed biotechnology companies, pharmaceutical companies, and research institutes.

What Makes Them Best for Cell Line Authentication Tests in India?

They have been successfully offering Cell Line Authentication Tests in India for many years. They carry out the Cell Line Authentication at their advanced facility, equipped with the latest technology and industry-standard methodologies. They provide 100% accurate & dependable results. Authentication of cells becomes essential because the authenticity of the research results greatly relies on this technology. 

They have the fastest turnaround times, providing the Cell Line Authentication Test report within 10-15 working days.

They also offer sample collection at the address of their customers. They are the only DNA testing company for a Legal DNA test in India for the courts of law.