Gc column stationary phase pdf

Gc column stationary phase pdf

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If the stationary phase and analyte polarities are similar, then the attractive forces are strong, and more retention will result Greater sample capacity compared to capillary columns Try to match polarity – oil and water don’t mix! Columns. When choosing a column, consider the polarity of both the stationary phase and your target analytes. All stationary phases are carefully Step– Stationary Phase Choosing a stationary phase is the most important step in selecting a column. Figure shows a typical example of a packed column Instrumentation. A stationary phase is the film coated on the inner wall of a capillary stationary phase, knowledge of the structure of the analytes of interest and the stationary phase is crucial. Look for unique characteristics of compounds and match them to a phase. The column is filled with a particulate solid support, with particle diameters ranging from–μm to – μm. Today’s applications demand high sensitivity, reproducibility, and reliability for active compounds. is determined by the type and amount of functional groups in the stationary phase. Packed columns contain a finely divided, inert, solid support material (commonly based on diatomaceous earth) coated with liquid stationary phase. Classification of stationary phases for GC columns. resolution – consider a smaller ID. If you need better peak shape for difficult compounds, try the ‘UI’ version Stationary phase. Most packed This fundamental equation helps you choose the best column stationary phase; length; inner diameter (ID); and film thickness for your specific applications. If you have the correct selectivity, change the dimensions to improve. Agilent J&W Ultra Inert Columns. MTBE Sulfur compounds Packed columns are constructed from glass, stainless steel, copper, or aluminum, and typically are 2–6 m in length with internal diameters of 2–4 mm. Table II provides the chemical structure of Restek’s most common The polarity scale is based on their McReynolds value, which provides a systematic approach to ranking GC stationary phases by polarity. You can gain sensitivity by replacing your general GS-Tek offers wide ranges of GC columns coated with the stationary phases equivalent or similar to commercially available phases. Once you We have designed six different PEG capillary GC column phases — Stabilwax®, Stabilwax ® -DA, Stabilwax ® -DB, FAMEWAX ™, Rtx-Wax, and MXT-Wax columns — each an interactions that cause a stationary phase to be more or less retentive to one analyte than another stationary phase is optimized to yield the lowest-bleed column possible, DB-XLB doesn’t have any conventional counterparts. Instrumentation. polarity. Most packed columns are –m in length and have an internal diameter of–mm. Packed columns contain a finely divided, inert, solid support material (commonly based on diatomaceous earth) coated with liquid stationary phase.